P-glu-d-phe-t rp-ser-tyr-d-ala-leu-arg-pro-gly-nh2-and intermediates

ABSTRACT

D-Phe2-D-Ala6-LRF, is described as well as its synthesis by solid phase techniques and novel intermediates formed by such synthesis. The novel decapeptide exhibits anti-ovulatory activity in mammals.

United States Patent Foell et -al.

[451 Dec. 17, 1974 P-GLU-D-PHE-T RP-SER-TYR-D-ALA-LEU-ARG-PRO-GLY- NHZ-AND INTERMEDIATES Inventors: Theodore J. F oell; John P. Yardley,

both of King of Prussia, Pa.

Assignee: American Home Products Corporation, New York, NY.

Filed: Oct. 3, 1973 Appl. No; 402,958

US. Cl. 260/112.5, 424/177 Int. Cl... C07c 103/52, C07g 7/00, A6lk 27/00 Field of Search 260/1 12.5

Primary ExaminerLewis Gotts Assistant Examiner-Reginald J. Suyat 5 7 ABSTRACT D-lhe -D-Ala -LRF, is described as'well as its synthesis by solid phase techniques and novel intermediates formed by such synthesis. The novel decapeptide exhibits anti-ovulatory activity in mammals.

8 Claims, No Drawings P-GLU-D-PHEPT RP-SER-TYR-D-ALA-LEU-ARG-PRO-GLY- NHZ-AND INTERMEDIATES This invention relates to the novel decapeptide p- Glu-D-Phe-Trp-Ser-Tyr-D-Ala-Leu-Arg-Pro-Gly-NH its process of manufacture and novel intermediates formed in such synthesis.

The luteinizing hormone releasing factor (hereafter called LRF) is the decapeptide, L-(5-oxoprolyl)-L- histidyl-L-tryptophyl-L-seryl-L-tyrosyl-glycyl-L-leucyl- L-arginyl-L-prolyl-glycine amide. This decapeptide is secreted by the hypothalamus and carried to the adenohypophysis where it stimulates the release of the luteinizing hormone and the follicle stimulating hormone. The present invention concerns itself with structural modifications of LRF which exhibit anti-ovulatory activity.

The novel peptides of the present invention are represented by the compounds of the formula:

p-Glu-D-Phe-Trp-Ser-Tyr-D-Ala-Leu-Arg-Pro-Gly- NH and its non-toxic salts. All chiral amino acid residues identified in formula 1 supra, and the other formulas hereinafter are of the natural or L-configuration unless specified otherwise.

Also contemplated within the scope of the present invention are intermediates of the formula wherein:

R is selected from the class consisting of NH OH, O-(lower)alkyl, in which (lower)alkyl is C through C (e.g. methyl, ethyl, pentyl, hexyl, etc.) and O-benzyl;

N means the side chain nitrogen atoms of arginine;

R is a protecting group for the N N and N"" nitrogen atoms of arginine selected from the class consisting of nitro, tosyl, benzyloxycarbonyl, and adamantyloxycarbonyl; or R is hydrogen which means there are no protecting groups on the side chain nitrogen atoms of arginine. Where the protecting group is nitro or tosyl, the protection is on either one of the N N nitrogens and in the case of benzyloxycarbonyl, or adamantyloxycarbonyl, the protection is on the N nitrogen and either one of the N N nitrogen atoms. The preferred protecting group defined by R is nitro;

R is a protecting group for the phenolic hydroxyl group of tyrosine selected from the class consisting of acetyl, tosyl, benzoyl, tetrahydropyranyl, tert-butyl, trityl, benzyl, 2,4 dichlorobenzyl and benzyloxycarbonyl. The preferred protecting group is benzyl; or R is hydrogen which means there is no protecting group on the phenolic hydroxy function;

R is at protecting group for the alcoholic hydroxyl group of serine and is selected from the class consisting of those members defined by R or R is hydrogen which means there is no protecting group on the alcoholic oxygen atom. Preferably R is benzyl;

R is preferably hydrogen or an a-amino protecting group. The a-amino protecting group contemplated by R are those known to be useful in the art in the stepwise synthesis of polypeptides. Among the classes of a-amino protecting groups covered by R are (1) acyl type protecting groups illustrated by the following: formyl, trifluoroacetyl, phthalyl, toluenesulfonyl (tosyl), benzensulfonyl, nitrophenylsulfenyl, tritylsulfeny], o-nitrophenoxyacetyl, chloroacetyl, acetyl, 'y-chlorobutyryl, etc.; (2) aromatic urethan type protecting groups illustrated by benzyloxycarbonyl and substituted benzyloxycarbonyl such as p-chlorobenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl; (3) aliphatic urethan protecting groups illustrated by tertbutyloxycarbonyl, diisopropylmethoxycarbonyl, isopropyloxycarbonyl, ethoxycarbonyl, allyloxycarbonyl; (4) cycloalkyl urethan type protecting groups illustrated by cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl; (5) thio urethan type protecting groups such as phenylthiocarbonyl; (6) alkyl type. protecting groups as illustrated by triphenylmethyl (trityl), benzyl; (7) trialkylsilane groups such as trimethylsilane. The preferred a-amino protecting group defined by R are selected from the class consisting of tert-butyloxycarbonyl, cyclopentyloxycarbonyl, tertamyloxycarbonyl and d-isobornyloxycarbonyl In formula II at least one of R, R or R is a protecting group.

A further aspect of the present invention relates to intermediates linked to a solid resin support. These intermediates are represented by the formula:

(III) wherein:

R, R and R have the same meaning as in Formula ll;

R is selected from the class consisting of R*-p-Glu, an a-amino protecting group or hydrogen (deprotected a-amino group). The a-amino protecting group is preferably terbbutyloxycarbonyl (tert-Boc) which protecting group is also preferably used for protecting the a-amino group of all amino acids added in the stepwise solid phase synthesis. However, other ot-amino protecting groups may be used such as o-nitrophenylsulfenyl, t-amyloxycarbonyl and biphenylisopropyloxycarbonyl;

A is an anchoring bond used in solid phase synthesis linked to a solid resin support. A is selected from the class consisting of:

resin resin N( J and OCHa (III (IIIb) The symbol means phenyl."

In selecting a particular side chain protecting group to be used in the synthesis of the peptides of formula (I), the following rules should be followed: (a) the protecting group must be stable to the reagent and under the reaction conditions selected for removing the a-amino protecting group at each step of the synthesis, (b) the protecting group must retain its protecting properties (i.e., not be split off under coupling conditions), and (c) the side chain protecting group must be removable upon the completion of the synthesis containing the desired amino acid sequence under reaction conditions that will not alter the peptide chain.

Illustrative of pharmaceutally acceptable non-toxic salts of formula 1 are hydrochloride, hydrobromide, sulfate, phosphate, maleate, acetate, citrate, benzoate, succinate, malate, ascorbate, and the like.

The peptides of formula (I) are prepared using solid phase synthesis. The synthesis is commenced from the C-terminal end of the peptide using an oz-amino protected resin. Such a starting material can be prepared by attaching an a-amino protecting glycine to a benzyhydrilamine resin, a chloromethylated resin or a hydroxymethyl resin, the former being preferred. The preparation of a benzhydrilamine resin is described by P. Rivaille et al., Helv. 54, 2772 (1971 and the preparation of the hydroxymethyl resin is described in B- danszky et al., Chem. Ind (London) 38, 1597-98 (1966). A chloromethylated resin is commercially available from Bio Rad Laboratories Richmond, California. In using the benzhydrilamine resin an amide anchoring bond is formed with the a-amino protected glycine as follows:

This permits the C-terminal amide function to be obtained directly after the amino acid sequence in the synthesis is complete by cleaving off the resin support to form the glycine amide at the C-terminal portion of the desired peptide of formula (I). When the other resins are used, the anchoring bond is the benzylester group as defined supra in Formula (Ilb), which after cleavage of the peptide from the resin support must be converted to the C-terminal amide. The preferred procedure is to ammonalyse the protected peptide off the resin and then remove the protecting group by hydrogenolysis or by hydrogen fluoride cleavage. An alternate procedure would be to cleave by transesterification with methanol/(EthN and then convert the resulting ester into an amide and subsequently deprotectect as described above. See .I. M. Stewart Solid Phase Peptide Synthesis," pg. 4246 (W. H. Freeman & Co. 1969).

The a-amino protected glycine is coupled to the benzhydrilamine resin with the aid of the carboxyl group activating compound such as dicyclohexylcarbodiimide. Following the coupling of the a-amino protected glycine to the resin support, the a-amino protecting group is removed such as by using trifluoroacetic acid in methylene chloride, trifluoroacetic acid alone or HCl in dioxane. The deprotection is carried out at a temperature between about 0C and room temperature. Other standard cleaving reagents and conditions for removal of specific a-amino protecting groups may be used as described in Schroder & Lubke, The Peptides, 1 7275 (Academic Press 1965). After removal of the a-amino protecting group the remaining a-amino protected amino acids are coupled step-wise in the desired order to obtain a compound of formula (1). However, as an alternate to adding each amino acid separately to the reaction, some of them may be coupled prior to addition to the solid phase reactor. If the C-terminal end of the peptide unit is represented by glycine or proline and the coupling is carried out with DCC, a minimum of racemization is encountered with proline and no problems are encountered with glycine which has no asymetric centre. Each protected amino acid or amino acid sequence, is introduced into the solid phase reactor in about a four-fold excess and the coupling is carried out in a medium of dimethylformamide: methylene chloride (1:1) or in dimethylformamide or methylene chloride alone. In cases where incomplete coupling occurred the coupling procedure is repeated before removal of the a-amino protecting group, prior to the coupling of the next amino acid to the solid phase reactor. The success of the coupling reaction at each stage of the synthesis is monitored by the ninhydrin reaction as described by E. Kaiser et al., Analyt. Biochem, 34, 595 1970).

After the desired amino acid sequence has been synthesized, the peptide is removed from the resin support by treatment with a reagent such as hydrogen fluoride which not only cleaves the peptide from the resin but also cleaves all remaining side chain protecting groups and the a-amino protecting group (if present) on pyroglutamic acid to obtain directly a compound of formula I in the case where the benzhydrilamine resin was used. Where a chloromethylated resin is used the peptide may be separated from the resin by methanolysis after which the recovered product is chromatographed on silica gel and the collected fraction subject to ammonalysis to convert the methyl ester to the C-terminal amide. Any side chain protecting group may then be cleaved as previously described or by other procedures such as catalytic reduction (e.g., Pd on C) using conditions which will keep the Trp moiety intact. When using hydrogen fluoride for cleaving, anisole is included in the reaction vessel to prevent the oxidation of labile amino acid (eg tryptophan).

The solid phase synthesis procedure discussed supra is well known in the art and has been essentially described by M. Monahan et al., C. R. Acad. Sci, Paris, 272, 508 (1971).

The nomenclature used for peptides is described by Schroder & Lubke, supra, pp viii-xxix and in Biochemistry 11, 1726-1732 (1972).

The following examples are illustrative of the preparation of the compounds of formulas I through 111.

EXAMPLE 1 L-Pyroglutamyl-D-phenylalanyl-L-tryptophyl-O- benzyl-L-seryl-O-benzyl-L-tyrosyl-D-alanyl-Lleucyl- N ""-NO -L-arginyl-L-prolylglycyl benzhydrilamine resin Benzhydrylamine resin (20.0 g.) is put in a Merrifield vessel of 300 ml. capacity and put through the following wash cycle: a) methylene chloride; b) trifluoroacetic acid (3 times for 10 minutes each); c) methylene chloride; d) methanol; e) triethylamine 12.5% in dimethylformamide (2 times for 10 minutes each); f) methanol (two times); g) methylene chloride (two times), allowing a contact time of at least 3 minutes each, if not indicated otherwise.

The resin so prepared is then gently shaken with tbutyloxycarbonyl glycine (3.65 g., 21 mmole), in 1:1 methylene chloridedimethylformamide and 25.6 ml. of 1M dicyclohexylcarbodiimide in methylene chloride is added in three portions over a period of thirty minutes. Shaking is continued at ambient temperature for a total of 18 hours. The peptide-resin is then washed successively with methanol, methylene chloride, methanol (twice), and methylene chloride (twice). To test for completeness of reaction, the peptide-resin is subjected to a ninhydrin test following the procedure of E. Kaiser et al., Analytical Biochemistry 34, 595 (1970).

The deprotection of the attached amino acid is carried out as follows: The peptide-resin is treated with a 1:1 solution of trifluoroacetic acid-methylene chloride (three times for minutes each), then steps (c) through (g), as described above for the wash cycle are performed. Again, a sample of the peptide-resin is subjected to a ninhydrin test to check for completeness of reaction. The sample is now strongly positive indicating deprotection of the glycine molecule attached to the resin.

The following amino acid residues are then introduced consecutively: t-Boc-L-proline (21 mmoles, 25.6 mmoles DCC), t-Boc-nitro-L-arginine (21 mmoles, 25.6 mmoles DCC), t-Boc-L-leucine (21 mmoles, 25.6 mmoles DCC), t-Boc-D-alanine (21 mmoles, 25.6 mmoles DCC) and t-Boc-O-benzyl-L-tyrosine (21 mmoles, 25.6 mmoles DCC). Each coupling step is carried out in a medium of methylene chloride dimethylformamide (1:1) and the removal of the a-amino protecting group at each step is performed as described for the deprotection of the t-Boc-glycine-resin.

At this point, the washed hexapeptideqesin is dried, weighed (36.0 g.), and the synthesis continued with one half (18.0 g.) of the hexapeptide-resin. The next amino acid added is t-BOC-O-benzyl-L-serine (10.4 mmoles, 13 mmoles DCC), followed by deprotection then t-BOC-L-tryptophan, (10.4 mmoles, 13 mmoles DCC). The washed peptide-resin is again dried (20.8 g. and the synthesis is continued with 2.0 g. of the peptide-resin. However, after the. addition of t-BOC- tryptophan the deprotection reaction is carried out with the addition of 5% 1,2-ethanedithiol added to the trifiuoroacetic acid-methylene chloride medium. Next t-BOC-D-phenylalanine 1.36 mmoles, 2 mmoles DCC), is added, then L-2-pyrrolidone-S-carboxylic acid, (2.7 mmoles, 3.4 mmoles DCC). The washed peptide-resin is dried in vacuo.

EXAMPLE 2 Pyroglutamyl-D-phenylalanyl-L-tryptophyl-L-seryl-L- tyrosyl-D-alanyl-L-leucyl-L-arginyl-L- prolylglycinamide Removal of the protecting groups and cleavage of the decapeptide from the resin is accomplished by treating the dried peptide-resin of Example 1 in vacuo with liquid hydrogen fluoride (25 ml.) and anisole (10 ml.) at ice bath temperature for one hour. The hydrogen fluoride is removed by vacuum distillation and the anisole removed by washing with ether. The peptide is dissolved in 10 percent acetic acid and removed from the resin by filtration. Lyophilization yields the desired decapeptide as a white fluffy powder.

EXAMPLE 3 Purification and characterization of Pyroglutamyl-D- phenylalanyl-L-tryptophyl-L-seryl-L-tyrosyl-D-alanyl- L-leucyl-L-arginyl-prolylglycinamide acetate The crude peptide is dissolved in a minimum volume of 0.2N acetic acid, applied to a Bio-Gel P-2 200400 mesh gel filtration column (2.6 cm x 90 cm) and eluted with the same solvent. Fractions of 9 ml. each are collected. The fractions containing the desired peptide are located by Pauly spot test and UV analysis. After pooling and lyophilization, a white fluffy powder (252 mg.) is obtained.

A partition column of Sephadex G-25 fine (2.6 cm x cm is prepared by equilibration with lower phase and then upper phase of the BAW solvent system (nbutanolzacetic acidzwater, 4:1:5, V ml.).

The lyophilized peptide from above is applied in a minimum volume of upper phase. Elution with upper phase 6 ml. fractions) affords the desired product which is located as described above. After pooling and lyophilization, a white fluffy powder 142.8 mg.) is obtained.

The optical rotation is measured on a Carl Zeiss LEP A-2 photoelectric precision polarimeter, [01], -36.54(c=1.024, 1% acetic acid); Amino acid analysis gives the following ratios: Ser (0.55), Glu (1.04), Pro (0.88), Gly 1.00), Ala 1.02), Leu (0.96 Tyr (1.02), Phe 1.04), N11 (0.68), Trp (0.65), Arg (1.04); R,(on silica): 0.28 (n-butanol:acetic acid:water, 421:5;

The peptide (20 ,u load) is homogeneous in three TLC systems (acidic, neutral, and basic) when examined under ultraviolet light, iodine vapor, and Pauly reagent.

The compounds of formula I possess anti-ovulatory activity and hence are potentially useful in inhibiting fertility in female mammals. ln tests conducted with female rats (225 to 250 grams body weight) complete ovulation inhibition was achieved at a dose of 12 mg/kg. The test was conducted with mature Sprague- Dawley rats, normally cycling, unanesthetized, proestrous rats. On the afternoon of proestrous, each rat in the test group received six subcutaneous injections of the acetate salt of formula 1 in corn oil, each injection being given a half hour following the previous injection. The rats are sacrificed the next morning and the number of animals ovulating and the number of ova shed are recorded following the procedure described by E. S. France, Neuroendocrinology 6, pp 77-89 (1970). The absence of or a significant decrease in the number of ova is the criterion for an anti-ovulation effect. At a dose of 500 ,1. per injection complete inhibition of ovulation was achieved.

The compounds of formula 1 can be administered to mammals intravenously, subcutaneously, intramuscularly or orally for fertility inhibition and control. The effective dosage will vary with the form of administration and the particular species of mammal to be treated. A typical dosage is a physiological saline solution containing a compound of formula I administered in a dose range of between about 8 to 12 mg/kg of body weight. Oral administration may be in either solid or liquid form.

What is claimed is:

1. A compound selected from the group consisting of L-p-Glu-D-Phe-L-Trp-L-Ser-L-Try-D-Ala-L-Leu-L- Arg-L-Pro-Gly-NH and R -L-P-Glu-D-Phe-L-Trp-L-Ser( R )-L-Tyr-( R )-D- Ala-L-Leu-L-Arg-(N -R )-L-Pro-Gly-R and its non-toxic salts; wherein R is selected from the class consisting of Nl-l OH, O-(lower)-alkyl and O-benzyl; R is a protecting group for the N N" and N nitrogen atoms of arginine selected from the class consisting of nitro, tosyl, benzyloxycarbonyl, and adamantyloxycarbonyl; or R is hydrogen;

R is a protecting group for the phenolic hydroxyl group of tyrosine selected from the class consisting of acetyl, tosyl, benzoyl, tert-butyl, tetrahydropyranyl, trityl, benzyl, 2,4 dichlorobcnzyl and benzyloxycarbonyl or R is hydrogen;

R is a protecting group for the alcoholic hydroxyl group of serine and is selected from the class consisting of acetyl, tosyl, benzoyl, tetrahydropyranyl, tert-butyl, trityl, 2,4 dichlorobenzyl, benzyl and benzyloxycarbonyl; or R is hydrogen;

R is selected from the class consisting of hydrogen or an a-amino protection group, with the proviso that at least one of R, R and R is a protecting group.

2. A compound according to claim 1 wherein R is 3. A compound according to claim 1 wherein R is NH R is nitro, R is benzyl, R is benzyl and R is hydrogen.

4. A compound according to claim 1 which is selected from: L-Pyroglutamyl-D-phenylalanyl-L- tryptophyl-L-seryl-L-tyrosyl-D-alanyl-L-leucyl'L- arginyl-L-prolyl-glycinamide and its non-toxic acid addition salts.

5. A compound of the formula:

wherein:

R, R and R have the same meaning as in claim 1;

R is selected from the class consisting of R -p-Gluan a-amino protecting group or hydrogen; and A is selected from the class consisting of nitro and R and R are benzyl.

" I I UNITED STATES PATENT OFFICE CERTIFICATE OF CORRECTION Patent No. 3,855,199 Dated December 17, 1974 Invent0r(s) .0 John P. Yardlev It is certified that error appears in the above-identified patent and that said Letters Patent are hereby corrected as shown below:

Column 4, line 4, change "asymetric" to -asymmetric--;

Column 6 (claim 1, line 2) change "Try" to i -i Column 8 (claim 5, line 2) before "Ser" insert,

(claim 5, line 5) delete "and R and insert --R and R Signed and sealed this 29th day of April 1975.

(SEAL) Attest:

C. MARSHALL DANN RUTH C. MASON Commissioner of Patents Attesting Officer and Trademarks 

1. A COMPOUND SELECTED FROM THE GROUP CONSISTING OF L-P-GLY-D-PHE-L-TRP-L-SER-L-TRY-D-ALA-L-LEU-L-ARG-LPRO-GLY-NH2 AND R4-L-P-GLU-D-PHE-L-TRP-L-SER(R3(-L-TYR(R2)-D-ALA-LLEU-L-ARG-(NG-R1)-L-PRO -GLY-R AND ITS NON-TOXIC SALTS; WHEREIN R IS SELECTED FROM THE CLASS CONSISTIG OF NH2, OH, O(LOWER)-ALKYL AND O-BENZYL THE N$, NW, AND NW'' NITROGEN ATOMS OF ARGININE SELECTED FROM THE CLASS CONSISTING OF NITRO, TOSYL, BENZYLOXYCARBONYL, AND ADAMANTHYLOXYCARBONYL; OR R1 IS HYDROGEN; R2 IS A PROTECTING GROUP FOR THE PHENOLIC HYDROXYL GROUP OF TYROSINE SELECTED FROM THE CLASS CONSISTING OF ACETYL, TOSYL, BENZOYL, TERT-BUTYL, TETRAHYDROPYRANYL, TRITYL, BENZYL, 2,4 DICHLOROBENZYL AND BENZYLOXYCARBONYL OR R2 IS HYDROGEN; R3 IS A PROTECTING GROUP FOR THE ALCOHOLIC HYDROXYL GROUP OF SERINE AND IS SELECTED FROM THE CLASS CONSISTING OF ACETYL, TOSYL, BENZOYL, TETRAHYDROPYRANYL, TERT-BUTYL, TRITYL, 2,4 DICHLOROBENZYL, BENZYL AND BENZYLOXYCARBONYL; OR R3 IS HYDROGEN; R4 IS SELECTED FROM THE CLASS CONSISTING OF HYDROGEN OR AN A-AMINO PROTECTION GROUP, WITH THE PROVISO THAT AT LEAST ONE OF R1, R2 AND R3 IS A PROTECTING GROUP.
 2. A compound according to claim 1 wherein R is NH2.
 3. A compound according to claim 1 wherein R is NH2, R1 is nitro, R2 is benzyl, R3 is benzyl and R4 is hydrogen.
 4. A compound according to claim 1 which is selected from: L-Pyroglutamyl-D-phenylalanyl-L-tryptophyl-L-seryl-L-tyrosyl-D-alanyl-L -leucyl-L-arginyl-L-prolyl-glycinamide and its non-toxic acid addition salts.
 5. A compound of the formula: R5-D-Phe-L-Trp-Ser(R3)-L-Tyr(R2)-D-Ala-L-Leu-L-Arg(NG-R1)-L-Pro-Gly-A wherein: R1, R2 and R3 have the same meaning as in claim 1; R5 is selected from the class consisting of R4-p-Glu-, an Alpha -amino protecting group or hydrogen; and A is selected from the class consisting of
 6. A compound according to claim 5 wherein R5 is an Alpha -amino protecting group which is selected from the class consisting of tert-butyloxycarbonyl, o-nitrophenylsulfenyl, tert-amyloxycarbonyl and biphenylisopropyloxycarbonyl;
 7. A compound according to claim 5 wherein A is a benzhydrilamine resin and R5 is R4-p-Glu and R4 is hydrogen.
 8. A compound according to claim 7 wherein R1 is nitro and R2 and R3 are benzyl. 